Lablisa® Human KLK5(Kallikrein 5) ELISA Kit

Product Information

Lablisa® Human KLK5(Kallikrein 5) ELISA Kit

Catalog No : LAB1203| Pack Size : 48T , 96T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLK5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK5 in the samples is then determined by comparing the OD of the samples to the standard curve.

Product name:Human KLK5(Kallikrein 5) ELISA Kit
Reactivity:Human
Alternative Names:KLK-L2; KLKL2; SCTE; Kallikrein-Related Peptidase 5; Kallikrein-like protein 2; Stratum corneum tryptic enzyme
Assay Type:Sandwich
Sensitivity:12.5 pg/mL
Standard:2000 pg/mL
Detection Range:31.25-2000 pg/mL
Sample Type:serum, plasma, saliva, tissue homogenates and other biological fluids
Assay Length:3.5h
Research Area:Enzyme & Kinase;Tumor immunity;


Standard curve



Concentration (pg/mL)ODCorrected OD
2000.002.2662.171
1000.001.5631.468
500.001.1211.026
250.000.9750.880
125.000.5090.414
62.500.2360.141
31.250.2130.118
0.000.0950.000


Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.


Recovery

Matrices listed below were spiked with certain level of recombinant KLK5 and the recovery rates were calculated by comparing the measured value to the expected amount of KLK5 in samples.

MatrixRecovery rangeAverage
serum(n=5)82-95%88%
EDTA plasma(n=5)95-107%101%
Heparin plasma(n=5)84-97%91%


Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KLK5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix1:21:41:81:16
serum(n=5)95-103%99-106%78-92%83-97%
EDTA plasma(n=5)86-97%90-98%80-93%92-105%
Heparin plasma(n=5)88-97%85-101%86-98%96-107%


Manuals on the web are for your reference only, specifications are subject to the delivery manuals.