Lablisa® Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Anti-Tissue Transglutaminase IgG |
| Assay Type: | Sandwich |
| Sensitivity: | 0.6 ng/mL |
| Standard: | 100 ng/mL |
| Detection Range: | 1.56-100 ng/mL |
| Sample type: | Serum, plasma, tissue homogenates and other biological fluids. |
| Assay length: | 3.5h |
| Research Area: | Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 100.00 | 2.035 | 1.940 |
| 50.00 | 1.663 | 1.568 |
| 25.00 | 1.121 | 1.026 |
| 12.50 | 0.815 | 0.720 |
| 6.25 | 0.585 | 0.490 |
| 3.13 | 0.549 | 0.454 |
| 1.57 | 0.203 | 0.108 |
| 0.00 | 0.095 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Anti-tTG IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-tTG IgG in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 82-94% | 88% |
| EDTA plasma(n=5) | 85-97% | 91% |
| Heparin plasma(n=5) | 83-95% | 89% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-tTG IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 83-97% | 82-96% | 86-98% | 93-101% |
| EDTA plasma(n=5) | 87-103% | 79-92% | 86-99% | 84-97% |
| Heparin plasma(n=5) | 88-97% | 95-106% | 83-98% | 93-105% |
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