Lablisa® Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit

Product Information

Lablisa® Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit

Catalog No : LAB0751 | Pack Size : 48T , 96T

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Product name:Lablisa® Human Anti-tTG IgG (Anti-Tissue Transglutaminase IgG) ELISA Kit
Reactivity:Human
Alternative Names:Anti-Tissue Transglutaminase IgG
Assay Type:Sandwich
Sensitivity:0.6 ng/mL
Standard:100 ng/mL
Detection Range:1.56-100 ng/mL
Sample type:Serum, plasma, tissue homogenates and other biological fluids.
Assay length:3.5h
Research Area:Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity;


Standard curve



Concentration (ng/mL)ODCorrected OD
100.002.0351.940
50.001.6631.568
25.001.1211.026
12.500.8150.720
6.250.5850.490
3.130.5490.454
1.570.2030.108
0.000.0950.000


Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.


Recovery

Matrices listed below were spiked with certain level of recombinant Anti-tTG IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-tTG IgG in samples.

MatrixRecovery rangeAverage
serum(n=5)82-94%88%
EDTA plasma(n=5)85-97%91%
Heparin plasma(n=5)83-95%89%


Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-tTG IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix1:21:41:81:16
serum(n=5)83-97%82-96%86-98%93-101%
EDTA plasma(n=5)87-103%79-92%86-99%84-97%
Heparin plasma(n=5)88-97%95-106%83-98%93-105%


Manuals on the web are for your reference only, specifications are subject to the delivery manuals.