Lablisa® Rat p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Rat p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | p-PRKAA1; p-AMPK; p-AMPKa1; p-AMPK alpha 1 |
| Assay Type: | Sandwich |
| Sensitivity: | 1.25 ng/mL |
| Standard: | 200 ng/mL |
| Detection Range: | 3.13-200 ng/mL |
| Sample type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay length: | 3.5h |
| Research Area: | Enzyme & Kinase |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 200.00 | 2.266 | 2.172 |
| 100.00 | 1.686 | 1.592 |
| 50.00 | 1.223 | 1.129 |
| 25.00 | 0.828 | 0.734 |
| 12.50 | 0.567 | 0.473 |
| 6.25 | 0.336 | 0.242 |
| 3.13 | 0.256 | 0.162 |
| 0.00 | 0.094 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant p-AMPK and the recovery rates were calculated by comparing the measured value to the expected amount of p-AMPK in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 83-98% | 90% |
| EDTA plasma(n=5) | 90-105% | 97% |
| Heparin plasma(n=5) | 87-95% | 91% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-AMPK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 79-92% | 87-103% | 89-98% | 80-101% |
| EDTA plasma(n=5) | 89-96% | 90-99% | 85-96% | 82-93% |
| Heparin plasma(n=5) | 91-105% | 87-98% | 99-102% | 89-102% |
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