Lablisa® Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit
Product Information
- Description
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Anti-tTG-IgA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-tTG-IgA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Anti-tTG-IgA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-tTG-IgA in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® Human Anti-tTG-IgA (Anti-Tissue Transglutaminase IgA) ELISA Kit |
Reactivity: | Human |
Alternative Names: | Anti-Tissue Transglutaminase IgA |
Assay Type: | Sandwich |
Sensitivity: | 1.45 IU/mL |
Standard: | 300 IU/mL |
Detection Range: | 4.69-300 IU/mL |
Sample Type: | Serum, plasma, tissue homogenates and other biological fluids. |
Assay Length: | 3.5h |
Research Area: | Enzyme & Kinase;Endocrinology;Neuro science;Autoimmunity; |
Standard curve
| Concentration (IU/mL) | OD | Corrected OD |
|---|---|---|
| 300.00 | 2.075 | 1.988 |
| 150.00 | 1.472 | 1.385 |
| 75.00 | 1.069 | 0.982 |
| 37.50 | 0.728 | 0.641 |
| 18.75 | 0.535 | 0.448 |
| 9.38 | 0.303 | 0.216 |
| 4.69 | 0.191 | 0.104 |
| 0.00 | 0.087 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Anti-tTG-IgA and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-tTG-IgA in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 85-97% | 91% |
| EDTA plasma(n=5) | 82-97% | 89% |
| Heparin plasma(n=5) | 87-95% | 91% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-tTG-IgA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-102% | 83-96% | 97-105% | 85-97% |
| EDTA plasma(n=5) | 78-91% | 86-92% | 89-96% | 91-97% |
| Heparin plasma(n=5) | 81-93% | 78-91% | 87-98% | 95-107% |
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