Lablisa® Mouse Phospho AKT S473 (p-AKT) ELISA Kit
Product Information
- Description
- More Infomation
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse p-AKT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse p-AKT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse p-AKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse p-AKT in the samples is then determined by comparing the OD of the samples to the standard curve.
Product name: | Lablisa® Mouse Phospho AKT S473 (p-AKT) ELISA Kit |
Reactivity: | Mouse |
Alternative Names: | p-AKT |
Assay Type: | Sandwich |
Sensitivity: | 49.6 pg/mL |
Standard: | 10000 pg/mL |
Detection Range: | 156.25-10000 pg/mL |
Sample Type: | tissue homogenates, cell lysates and other biological fluids |
Assay Length: | 3.5h |
Research Area: | Enzyme & Kinase; |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 10000.00 | 2.266 | 2.170 |
| 5000.00 | 1.587 | 1.491 |
| 2500.00 | 1.282 | 1.186 |
| 1250.00 | 0.806 | 0.710 |
| 625.00 | 0.559 | 0.463 |
| 312.50 | 0.357 | 0.261 |
| 156.25 | 0.236 | 0.140 |
| 0.00 | 0.096 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant p-AKT and the recovery rates were calculated by comparing the measured value to the expected amount of p-AKT in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 78-95% | 86% |
| EDTA plasma(n=5) | 89-105% | 97% |
| Heparin plasma(n=5) | 86-99% | 92% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p-AKT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 91-102% | 79-91% | 89-103% | 82-95% |
| EDTA plasma(n=5) | 79-92% | 87-103% | 79-96% | 92-105% |
| Heparin plasma(n=5) | 85-92% | 96-103% | 87-98% | 95-106% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
