Lablisa® Rat TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit
Product Information
- Description
- More Infomation
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat TPO-Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO-Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO-Ab in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Rat TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | TPO Ab; TPO-Ab |
| Assay Type: | Competitive Inhibition |
| Sensitivity: | 1.25 IU/mL |
| Standard: | 80 IU/mL |
| Detection Range: | 1.25-80 IU/mL |
| Sample Type: | serum, plasma, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 2h |
| Research Area: | Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology; |
Standard curve
| Concentration (IU/mL) | OD | Corrected OD |
|---|---|---|
| 80.00 | 0.191 | |
| 40.00 | 0.379 | |
| 20.00 | 0.567 | |
| 10.00 | 0.959 | |
| 5.00 | 1.152 | |
| 2.50 | 1.511 | |
| 1.25 | 1.835 | |
| 0.00 | 2.123 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant TPO-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of TPO-Ab in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 83-95% | 89% |
| EDTA plasma(n=5) | 87-99% | 93% |
| Heparin plasma(n=5) | 92-105% | 98% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TPO-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 87-103% | 97-105% | 79-90% | 87-96% |
| EDTA plasma(n=5) | 87-98% | 87-100% | 85-94% | 83-96% |
| Heparin plasma(n=5) | 85-100% | 84-98% | 95-101% | 88-102% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
