Lablisa® Rat IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit
Product Information
- Description
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The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IFABP/FABP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFABP/FABP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IFABP/FABP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IFABP/FABP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
| Product name: | Lablisa® Rat IFABP/FABP2(Intestinal Fatty Acid Binding Protein) ELISA Kit |
| Reactivity: | Rat |
| Alternative Names: | FABPI; IFABP; I-FABP; Intestinal-type fatty acid-binding protein; FABP2; Fatty Acid Binding Protein 2, Intestinal |
| Assay Type: | Sandwich |
| Sensitivity: | 0.139 ng/mL |
| Standard: | 20 ng/mL |
| Detection Range: | 0.32-20 ng/mL |
| Sample Type: | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Metabolic pathway;Tumor immunity;Endocrinology;Cardiovascular biology; |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 20.00 | 1.989 | 1.900 |
| 10.00 | 1.628 | 1.539 |
| 5.00 | 0.976 | 0.887 |
| 2.50 | 0.782 | 0.693 |
| 1.25 | 0.457 | 0.368 |
| 0.63 | 0.357 | 0.268 |
| 0.32 | 0.236 | 0.147 |
| 0.00 | 0.089 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant IFABP/FABP2 and the recovery rates were calculated by comparing the measured value to the expected amount of IFABP/FABP2 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 87-99% | 93% |
| EDTA plasma(n=5) | 82-95% | 88% |
| Heparin plasma(n=5) | 94-107% | 100% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFABP/FABP2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 88-97% | 96-105% | 81-96% | 93-107% |
| EDTA plasma(n=5) | 82-96% | 85-98% | 92-103% | 81-95% |
| Heparin plasma(n=5) | 95-102% | 83-96% | 97-105% | 85-97% |
Manuals on the web are for your reference only, specifications are subject to the delivery manuals.
